| Test Code | Test |
| 8574 | ABO phenotyping |
| 8500 | B virus |
| 8573 | Burkholderia pseudomallei |
| 8554 | Campylobacter screen (genus-specific) |
| 8556 | Campylobacter jejuni |
| 8579 | Chikungunya virus |
| 8501 | CMV (Cytomegalovirus) |
| 8541 | CMV African green monkey (SA6) |
| 8539 | EMCV (Encephalomyocarditis virus) |
| 8567 | Filovirus |
| 8561 | Helicobacter screen (genus-specific) |
| 8520 | Hepatitis B genotype A-H |
| 8548 | Hepatitis B genotype D |
| 8538 | Klebsiella pneumoniae rmpA/magA genotyping |
| 8557 | Lawsonia intracellularis |
| 8532 | Leptosporia |
| 8549 | Lymphocryptovirus |
| 8534 | Malaria |
| 8566 | Male Sex |
| 8535 | Mamu-A*01 screen |
| 8536 | Mamu-A*01 sequencing confirmation |
| 8582 | Measles RT |
| 8587 | MRSA (Methicillin-Resistant Staphylococcus aureus) |
| 8527 | Mycobacterium (genus-specific) |
| 8529 | Mycobacterium tuberculosis complex (MTC) |
| 8530 | M. tuberculosis and M. bovis |
| 8555 | Respiratory syncytial virus (RSV) |
| 8550 | Salmonella |
| 8551 | Shigella |
| 8531 | Salmonella/Shigella |
| 8504 | SFV(Simian foamy virus)-African green monkey |
| 8505 | SFV-Baboon |
| 8506 | SFV-Chimpanzee |
| 8507 | SFV-Macaque |
| 8508 | SIV(Simian immunodeficiency virus)-African green monkey, AGM-3 |
| 8510 | SIV-Chimpanzee |
| 8509 | SIV-Macaque |
| 8559 | SIV screen (genus-specific) |
| 8092 | Simian Parvovirus (SPV) |
| 8511 | SRV/D screen (SRV-1,2,3,4,5) |
| 8512 | SRV-1 (SRV/D subtype 1) |
| 8513 | SRV-2 (SRV/D subtype2) |
| 8514 | SRV-3 (SRV/D subtype 3) |
| 8553 | SRV-4 (SRV /D subtype 4) |
| 8515 | SRV-5 (SRV/D subtype 5) |
| 8516 | STLV-1 |
| 8517 | SV40 |
| 8552 | Simian Varicella Virus (SVV) |
| 8583 | Trypanosoma cruzi kDNA |
| 8540 | Trypanosoma cruzi nDNA |
| 8558 | Yersinia (genus-specific) screen |
| 8543 | Yersinia enterocolitica |
| 8542 | Yersinia pseudotuberculosis |
| 8577 | Yersinia pseudoturberculosis genotyping |
| 8581 | Zika virus |
Zika virus – RT-PCR
8581
0.5-1 ml serum or CSF (collect within 7 days of symptom onset), 0.5-1 ml urine (collect within 30 days of symptom onset), ship overnight with ice packs; 1 cm3 fresh frozen tissue, frozen as soon as possible at -70°C, ship overnight with dry ice.
This assay is used to diagnose Zika virus acute infection.
Yersinia pseudotuberculosis-real time PCR
8542
Stool in sterile screw-top container, rectal swab, bacterial isolate on solid media, EDTA whole blood, fresh or frozen leison tissues. Ship with cold packs.
This species-specific PCR only detects Yersinia enterocolitica but not other species.
Leptosporia-real time PCR
8532
2mL of EDTA or ACD whole blood, 10mL urine, 0.5mL CSF or tissue. Ship with cold packs.
This assay detects(but not differentiate) pathogenic Leptospira species in the early infection stage. Blood, CSF and tissue samples are suggested to be collected during the first week prior to seroconversion; urine samples are collected 7-14 days post infection.
Yersinia enterocolitica-real time PCR
8543
Stool in sterile screw-top container, rectal swab, bacterial isolate on solid media, EDTA whole blood, fresh or frozen leison tissues. Ship with cold packs.
This species-specific PCR only detects Yersinia enterocolitica but not other species.
Yersinia (genus-specific) screen- real time PCR
8558
Stool in sterile screw-top container, rectal swab, bacterial isolate on solid media, EDTA whole blood, fresh or frozen liver and spleen tissues. Ship with cold packs.
This assay detects (but not differentiates) all species in the genus Yersinia.
Trypanosoma cruzi nDNA (Chagas disease)-real time PCR
8540
2 ml EDTA or ACD whole blood, blood clot, serum. Fresh samples are preferred to be sent by overnight shipping with cold packs.
This assay targets satellite repeat of T. cruzi nuclear DNA (nDNA). The specificity of this test is higher than T. cruzi kinetoplast DNA (kDNA) PCR (test code: 8583). T. curzi is transmitted from triatomid insect vectors to vertebrate hosts and causes Chagas disease. Natural T.cruzi infection has been reported in many non-human primates, including Macaca sp., a baboon, great apes, squirrel monkeys, owl monkeys, marmosets, tamarins, spider monkeys, woolly monkeys, cebus monkeys and uakaris. T. cruzi is commonly spread between monkeys in the wild by reduviid bugs, but it also can be spread in captive monkey colonies by blood-to blood exposure, saliva, sexual activity and transplacental transmission. The infection of T. cruzi in non-human primates causes nonspecific clinical signs, such as fever, generalized edema without necrosis or hemorrhage, anemia, hepatosplenomegaly, lymphadenitis and myocarditis. The diagnosis of acute infection of T. cruzi can be made by identifying parasites by microscopic examination of fresh blood or by PCR. Serological screening of T. cruzi IgG antibodies can establish prior exposure to the infection.
Simian varicella virus (SVV) - PCR
8552
2 ml EDTA or ACD whole blood, lesion tissues, vesicle fluid or neural ganglia. Ship with cold packs.
This assay not only detects SVV DNA during lytic infection, but also detects latent infection in neural ganglia.
SV40 - PCR
8517
2 ml EDTA or ACD whole blood, or a T25 flask of cell culture, or 10 ml urine sample. Overnight shipping at ambient temperature or with cold packs for more than 24 hours transit.
Lawsonia intracellularis - PCR
8557
Stool in sterile screw-top container, rectal swab and fresh or frozen ileum tissue in sterile container. Ship on ice packs.
Lawsonia intracellularis is usually detected in early infection stage and prior to seroconversion in faeces.
STLV - 1 (simian T-cell leukemia virus type 1) - real time PCR
8516
2 ml EDTA or ACD whole blood. Ship with cold packs.
This assay only detects STLV-1, but not STLV-2 and STLV-3.
SRV-5 (SRV/D subtype 5) - PCR
8515
2ml EDTA or ACD whole blood. Ship with cold packs.
This assay specifically detects SRV-5.
SRV-4 (SRV/D subtype 4) - PCR
8553
2ml EDTA or ACD whole blood. Ship with cold packs.
This assay specifically detects SRV-4.
SRV-3 (SRV/D subtype 3) - PCR
8514
2 ml EDTA or ACD whole blood. Ship with cold packs.
This assay specifically detects SRV-3.
SRV-2 (SRV/D subtype 2) - PCR
8513
2 ml EDTA or ACD whole blood. Ship with cold packs.
This assay specifically detects SRV-2.
SRV-1 (SRV/D subtype 1) - PCR
8512
2 ml EDTA or ACD whole blood. Ship with cold packs.
This assay specifically detects SRV-1.
SRV (simian type D retrovirus) Screen - real time PCR
8511
2 ml EDTA or ACD whole blood. Ship with cold packs.
This assay detects but not differentiate SRV-1, 2, 3, 4 and 5. SRV/Ds are commonly found in genus Macaca and may cause simian acquired immune deficiency symdrome (SAIDS) in monkeys.
Simian Parvovirus (SPV) - PCR
8092
0.5 ml serum. Ship with cold packs.
This assay detects but does not differentiates simian parvovirus (SPV or cynomolgus macaque parvovirus), rhesus macaque parvovirus (RmPV), and pig-tailed macaque parvovirus (PMPV). Differentiation of SPV, RmPV and PMPV can be done upon request.
SIV screen (genus-specific) - PCR
8559
2 ml EDTA or ACD whole blood. Shipped with cold packs.
This assay detects (but not differentiates) proviral DNA of primate lentiviruses.
SIV Macaque - PCR
8509
2 ml EDTA or ACD whole blood shipped with cold packs.
This assay detects SIVmac.
SIV Chimpanzee - PCR
8510
2 ml EDTA or ACD whole blood. Ship with cold packs.
This assay detects SIVcpz.
SIV (Simian immunodeficiency virus) African green monkey, AGM-3 - PCR
8508
2 ml EDTA or ACD whole blood shipped with ice packs.
This assay detects SIVagm strain AGM-3.
SFV (Simian Foamy virus), Macaque - PCR
8507
2 ml EDTA or ACD whole blood. Ship with cold packs.
This assay detects SFVmac.
SFV (Simian Foamy Virus), Chimpanzee - PCR
8506
2 ml EDTA or ACD whole blood. Ship with cold packs.
This assay detects SFVcpz.
SFV (Simian Foamy Virus), Baboon - PCR
8505
2 ml EDTA or ACD whole blood. Ship with cold packs.
This assay detects SFVbab.
SFV (Simian Foamy Virus), African green monkey - PCR
8504
2 ml EDTA or ACD whole blood. Ship with cold packs.
This assay detects SFVagm.
Salmonella/Shigella - PCR
8531
Stool in sterile screw-top container, rectal swab or bacterial isolate on solid media. Ship with cold packs.
For Salmonella, this assay detects (but not differentiate) all Salmonella species. For Shigella, this assay detects all four Shigella species as well as enteroinvasive Escherichia coli (EIEC).
Shigella PCR
8551
Stool in sterile screw-top container, rectal swab or bacterial isolate on solid media. Ship with cold packs.
This assay detects all four Shigella species as well as enteroinvasive Escherichia coli (EIEC).
Salmonella PCR
8550
Stool in sterile screw-top container, rectal swab or bacterial isolate on solid media. Ship with cold packs.
This assay detects (but not differentiate) all Salmonella species.
Respiratory syncytial virus (RSV) - RT - PCR
8555
Nasal or nasopharyngeal swab or aspirate or wash. Ship with cold packs.
This assay detects but does not differentiates RSV subgroup A and B. Subgroup typing can be performed upon request.
Mycobacterium tuberculosis and Mycobacterium bovis - PCR
8530
Preferred Specimen: Lesion tissues, lymph nodes, 1ml bronchoalveolar lavage. Ship with cold packs. Less preferred specimen: 2 ml EDTA or ACD whole blood shipped with cold packs.
Detection (but not differentiation) of M. tuberculosis and M. bovis.
Mycobacterium tuberculosis complex (MTC) - real time PCR
8529
Preferred Specimen: Lesion tissues, lymph nodes, 1ml bronchoalveolar lavage. Ship with cold packs. Less preferred specimen: 2 ml EDTA or ACD whole blood shipped with cold packs.
This real time PCR assay detects (but not differentiate) MTC members, including M. tuberculosis, M. bovis, M. africanum, M. microti and M. canetti.
Mycobacterium screen (genus-specific) PCR
8527
Preferred Specimen: Lesion tissues, lymph nodes, 1ml bronchoalveolar lavage. Ship with cold packs. Less preferred specimen: 2 ml EDTA or ACD whole blood shipped with cold packs.
This genus-specific This genus-specific PCR detects all members in the genus Mycobacterium. This assay can not differentiate mycobacteria species.
Measles- RT-PCR
8582
Preferred Specimen: Thorat (oropharyngeal), nasal or nasopharyngeal swabs (collect samples within 14 days from rash onset). Other Acceptable Specimen: 5-10 ml urine or 2 ml EDTA whole blood (collect within 14 days from rash onset), 0.5-1 ml sera (collect within 14 days from rash onset).
This assay is used to detect measles RNA in the early infection stage.
Mamu-A*01 Sequencing Confirmation
8536
2 ml EDTA whole blood. Ship with cold packs.
Mamu-A*01 is a rhesus macaque (Macaca mulatta) MHC (major histocompatibility complex) class I allele which can present a peptide from the SIV (simian immunodeficiency virus) gag protein for stimulating CTLs (cytotoxic T lymphocytes). Expression of Mamu-A*01 is significantly associated with lower viral load and longer survival time in SIV infected rhesus macaques. Mamu-A*01 positive rhesus macaques infected with SIV are therefore often used as an AIDS model for pathogenesis study and vaccine evaluation. The frequency of Mamu-A*01 allele detected in Indian rhesus macaques is from 22.06 to 66.16%, while in Chinese rhesus macaques is from 0 to 3.85%.
Mamu-A*01 PCR Screen
8535
2 ml EDTA whole blood. Ship with cold packs.
Mamu-A*01 is a rhesus macaque (Macaca mulatta) MHC (major histocompatibility complex) class I allele which can present a peptide from the SIV (simian immunodeficiency virus) gag protein for stimulating CTLs (cytotoxic T lymphocytes). Expression of Mamu-A*01 is significantly associated with lower viral load and longer survival time in SIV infected rhesus macaques. Mamu-A*01 positive rhesus macaques infected with SIV are therefore often used as an AIDS model for pathogenesis study and vaccine evaluation. The frequency of Mamu-A*01 allele detected in Indian rhesus macaques is from 22.06 to 66.16%, while in Chinese rhesus macaques is from 0 to 3.85%.
Male Sex-PCR
8566
2 ml EDTA or ACD whole blood, maternal serum or plasma (EDTA) collected from pregnant monkeys. Ship with cold packs.
This assay detects Y-chromosomal DNA sequences and can be used for fetal sex determination from maternal blood.
Malaria - real time - PCR
8534
2 ml EDTA or ACD whole blood. Ship with cold packs.
PCR detects all members in genus Plasmodium. This assay can not differentiate Plasmodium species.
Lymphocryptovirus –real time PCR
8549
Oropharyngeal swabs, 2 ml EDTA or ACD whole blood. Ship with cold packs.
This assay is to detect rhesus and cynomolgus LCV DNA.
Klebsiella pneumoniae rmpA/magA genotyping PCR
8538
Stool/oral swab/ rectal swab or culture positive colony on solid media. Ship with cold packs.
This assay detects and differentiates rmpA and magA genes. These two virulence genes are associated with the hypermucoviscosity phenotype of K. pneumoniae. The presence of rmp A and magA have been documented to be relevant to the pathogenesis of K. pneumoniae liver abscess.
Hepatitis B (HBV) genotype D – real time PCR
8548
2 ml EDTA or ACD whole blood, 1 ml plasma or serum, or frozen liver tissue, ship with cold packs.
This assay is a qualitative detection of HBV genotype D.
Hepatitis B (HBV) genotype A-H – real time PCR
8520
2 ml EDTA or ACD whole blood, 1 ml plasma or serum, or frozen liver tissue, ship with cold packs.
This assay detects all of HBV genotypes (A-H).
Helicobacter screen (genus-specific)- real time PCR
8561
Fresh stool, fresh or frozen cecum or liver tissue shipped with cold packs.
This genus-specific PCR detects all members in the genus Helicobacter. This assay cannot differentiate Helicobacter species.
Filovirus-RT-PCR
8567
2 ml EDTA or ACD whole blood, sera, plasma, fresh or frozen tissues, mucosal secretions (nasal, oral, anal, vaginal swabs). Ship with cold packs.
Two genera, Ebolavirus and Marburgvirus, are in the Filoviridae family. There are 5 known Ebolavirus species, Zair ebolavirus, Sudan ebolavirus, Reston ebolavirus, Cote d’Ivoire ebolavirus, and Bundibugyo ebolavirus; whereas there is only a Marburgvirus species, Lake Victoria marburgvirus. This RT-PCR assay detects all filovirus species. Differentiation can be done upon request.
Encephalomyocarditis virus (EMCV)-RT-PCR
8539
Fresh heart, brain, spleen, liver, pancreas, and skeletal muscle tissues shipped with cold packs. Frozen tissues shipped on dry ice.
This assay is a qualitative detection of EMCV.
Cytomegalovirus in African green monkey (AgmCMV, SA6)-PCR
8541
2 ml EDTA or ACD whole blood, 0.5 ml serum/plasma, salvia, oral swabs, urine or tissues. Ship with cold packs.
This assay only detects African green monkey CMV (SA6) and doesn’t detect other CMV strains.
Cytomegalovirus - PCR
8501
2 ml EDTA or ACD whole blood, 0.5 ml serum/plasma, saliva, oral swabs, urine, or tissues. Ship with cold packs.
This assay detects CMV from humans, chimpanzees, baboons, African green monkeys (SA6) and macaques.
Chikungunya virus (CHIKV) -RT-PCR
8579
2 ml EDTA or ACD whole blood, 0.5 ml serum/plasma, fresh tissues shipped with cold packs; frozen tissues shipped with dry ice.
This assay is used to detect CHIKV during acute or recent infection (within 7 days post infection).
Campylobacter jejuni - PCR
8556
Stool in sterile screw-top container, rectal swab or bacterial isolate on solid media. Ship with cold packs.
This assay specifically detects Campylobacter jejuni.
Campylobacter (genus-specific) Screen - PCR
8554
Stool in sterile screw-top container, rectal swab or bacterial isolate on solid media. Ship with cold packs.
This is genus-specific PCR detects all members in the genus Campylobacter. This assay cannot differentiate Campylobacter species.
Burkholderia pseudomallei
8573
2 ml EDTA or ACD whole blood, biopsy tissue or swab. Ship with cold packs.
This assay specifically detects B. pseudomallei.
B virus
8500
Mucosal swab, CSF or tissue in suitable transport medium, shipped overnight with cold packs.
Whole blood samples are not recommended for B virus PCR.
ABO phenotyping
8574
2 ml EDTA or ACD whole blood. Ship with cold packs.
This is a SNP genotyping assay to determine A, B, or AB phenotype in rhesus or cynomolgus macaques. This assay does not detect O phenotype.
Trypanosoma cruzi kDNA (Chagas disease)-real time PCR
Yersinia pseudotuberculosis genotyping
Test Code
8577
Specimen Required
Yersinia pseudotuberculosis isolate on solid media.
Comments
This multiplex PCR is used for O-genotyping of 21 known Yersinia pseudotuberculosis serotypes.
MRSA PCR
Test Code
8587
Specimen Required
Nasal swabs placed in Amies Liquid Collection tube; Stuart’s Liquid Collection tube (such as BD BBL CultureSwab Transport Systems: Liquid Amies, Single Swab, cat# BD 220093; Copan Venturi Transystem Liquid Amies-Single Swab, cat# Copan 140C; Copan Venturi Transystem Liquid Stuart-Single Swab, cat# Copan 141C). Swabs from the groin, wounds, axilla, vagina, and throat are also acceptable. Culture positive colonies. Ship with cold packs.
Comments
Staphylococcus aureus causes a variety of diseases, such as skin and soft tissue infections, endocarditis, and septicemia. The emergence of methicillin resistant S. aureus (MRSA) is an increasing threat since they are resistant to multiple antibiotics and limit antibiotic treatment options. This real time PCR assay is used for MRSA screening.