VRL announces that we now have Zika virus RT-PCR available for testing non-human primate samples: Zika virus RT-PCR Order Code 8581 Specimen Requirements: 0.5-1 ml serum or CSF (collect within 7 days of symptom onset), 0.5-1 ml urine (collect within 30 days of symptom onset), ship overnight with ice packs; 1 cm3 fresh frozen tissue, frozen as soon as possible at -70°C, ship overnight with dry ice. Zika virus is an emerging mosquito-born virus in the genus Flavivirus. It was first discovered from a rhesus monkey in the Zika forest of Uganda in 1947. The outbreaks in humans have been reported in Africa, Southeast Asia and the Pacific Islands. Since [...]
Dear Clients, We are pleased to inform you that to accommodate the growing demand of our testing services, VRL-Maryland will be relocating our labs to a bigger and newer space. We will continue to offer the same dedicated services and quality. Please note that beginning on 01 August 2015, all samples should be shipped to our new address listed below: 401 Professional Drive Suite 210 Gaithersburg, Maryland 20879 For inquiries please call 301-610-2521 or email us at firstname.lastname@example.org or visit us at www.vrl.net We would like to thank you in advance for your patience with this transition. Thank you for your business!
VRL announces that we now have T. cruzi PCR available for testing canine samples: T.cruzi PCR Test Code 8540 Specimen Requirements: 1.0 ml-2.0 ml EDTA whole blood Chagas disease is a parasitic infection caused by T. curzi, which is transmitted by triatomid insect vectors to vertebrate hosts. Natural T.cruzi infection has been reported in humans, primates, live stocks, pets, rodents, etc. Chagas disease is endemic in South America, Central America, U.S. and Mexico. Seroprevalence of T. cruzi in dogs is about 7.5-8.8% in Texas, U.S. The infection of T. cruzi in dogs causes nonspecific clinical signs, such as depression, lethargy, exercise intolerance, and increased heart rate. Some dogs may be asymptomatic and progressively develop heart failure in years. The [...]
VRL 7540 Louis Pasteur Drive Suite 200 San Antonio, TX 78229 Date : December 31, 2013 Dear all, We are pleased to announce that we have recently acquired the business assets of BioReliance Animal Health Services based in Rockville, MD. BioReliance Animal Health Services have been a scientific leader in laboratory animal diagnostics for over 60 years. BioReliance Animal Health Services testing programs are used world-wide by breeding colonies, animal facilities, pharmaceutical/biotech and universities. The acquisition of BioReliance Animal Health Services will give us a stronger presence in not only the simian diagnostic testing arena but also the small animal testing world. We believe the two combined companies [...]
Zao CL, Tomanek L, Hurtado-McClure G, Cooke A, Berger R, Boulineau TM, Turner OC, Covington DE. Abstract Fatal Yersinia pseudotuberculosis infection in cynomolgus macaques was diagnosed based upon pathology, microbiology and PCR for this study. Pathological findings included acute, erosive to ulcerative, necrohemorrhagic enterocolitis. Genotyping by PCR showed an O:3 pattern (gmd-fcl(+), ddhC-prt(+), manB(+), ddhA-B(+)), but an additional gene, wbyK, was detected. This is the second report to identify wbyK+ O:3 genotype as the cause of fatal yersiniosis. The first case was reported in 2008, and involved farm deer in the U.S. As the frequency of wbyK+ O:3 genotype is found more often in different carriers, O:3 genotype [...]
VRL is pleased to announce effective May 1, 2012 the launch of new ***Chemistry and CBC Testing Profiles*** 5500 – Chem Profile I Specimen Requirements: 0.5 – 1.0 mL serum Albumin, Alkaline Phosphatase, Alanine Aminotransferase (ALT, SGPT), Amylase, Blood Urea Nitrogen (BUN), Calcium, Cholesterol, Creatinine, Globulin, Glucose, Phosphorus, Total Bilirubin, Total Protein. 5501 – Chem Profile II Specimen Requirements: 0.5 – 1.0 mL serum Albumin/Globulin Ratio (A/G Ratio), Albumin, Alkaline Phosphatase, Alanine Aminotransferase (ALT, SGOT), Amylase, Aspartate Aminotransferase (AST, SGOT), BUN Creatinine Ratio (B/C Ratio), Blood Urea Nitrogen (BUN), Calcium, Chloride, Cholesterol, Creatinine Phosphokinase (CPK), Creatinine, Gamma Glutamyl Transferase (GGT), Globulin, Glucose, Lipase, Magnesium, Na/K Ratio, Phosphorus, Potassium, Sodium, Total Bilirubin, Total [...]
VRL annouces that we now have T. cruzi ELISA and PCR available for our clients. cruzi ELISA (Order code L154): Specimen requirements: 0.5 – 1.0 ml serum cruzi PCR (Order code 8540): Specimen requirements: 1.0 – 2.0 ml EDTA whole blood Chagas disease is a parasitic infection caused by T. curzi, which is transmitted by triatomid insect vectors to vertebrate hosts. Natural T.cruzi infection has been reported in many non-human primates, including Macaca sp., a baboon, great apes, squirrel monkeys, owl monkeys, marmosets, tamarins, spider monkeys, woolly monkeys, cebus monkeys and uakaris. T. cruzi is commonly spread between monkeys in the wild by reduviid bugs, but it also can be spread in captive monkey [...]
Characterization of SRV-4 infected cynomolgus monkeys” is published in Arch Virol 2011; 156 (11): 2053-6
Virological and serological characterization of SRV-4 infection in cynomolgus macaques Zao CL, Ward JA, Tomanek L, Cooke A, Berger R, Armstrong K. The nature of SRV-4 infection in cynomolgus macaques remains unclear to date. Here, we report the monitoring of 24 cynomolgus monkeys that were naturally infected with SRV-4 for virus isolation, proviral load and antibody. The results indicated that the SRV-4 antibody status was statistically correlated to environmental temperature.
The complete Genome and Genetic Characteristics of SRV-4 Isolated from Cynomolgus Monkeys (Macaca fascicularis) Zao CL, Armstrong K, Tomanek L, Cooke A, Berger R, Estep JS, Marx PA, Trask JS, Smith DG, Yee JL, Lerche NW. At least 5 serotypes of exogenous simian retrovirus type D (SRV/D) have been found in nonhuman primates, but only SRV-1, 2 and 3 have been completely sequenced. SRV-4 was recovered once from cynomolgus macaques in California in 1984, but its genome sequences are unknown. Here we report the second identification of SRV-4 and its complete genome from infected cynomolgus macaques with Indochinese and Indonesian/Indochinese mixed ancestry. Phylogenetic analysis demonstrated that SRV-4 was [...]
VRL announces that we now have 3 methodologies available for our clients, for the detection of SVV (Simian Varicella Virus) IgG antibodies: SVV ELISA (Test code: L111; Specimen requirements: 0.5 – 1.0 ml serum) SVV IFA (Test code: F111; Specimen requirements: 0.5 – 1.0 ml serum) SVV DIA (Test code: 111; Specimen requirements: 0.5 – 1.0 ml serum) Simian varicella virus (SVV) causes an erythematous varicella-like disease in Old World monkeys. SVV is closely related to varicella-zoster virus (VZV), which causes human varicella (chickenpox) in the primary infection and herpes zoster (shingles) in the reactivation stage. SVV is highly contagious and the transmission is through inhalation of aerosols [...]